"Examples of the many threatened species in this hotspot include three that are Critically Endangered and known from single localities: Phyllomedusa ayeaye, from Poços de Caldas in the state of Minas Gerais; Scinax alcatraz, from the Ilha de Alcatrazes in the state of São Paulo; and Hyla cymbalum, from Grande da Serra in the state of São Paulo (and which has not been recorded during recent surveys and may be extinct)."